ADAPTATION OF “DRIED BLOOD DROP” METHOD FOR THERAPEUTIC DRUG MONITORING

To control the concentration of drugs with a narrow therapeutic range, and to conduct effective safe treatments, Therapeutic Drug Monitoring (TDM) is carried out. However, date, implementation TDM associated various difficulties, for solution which more convenient less invasive methods collecting biological material are being developed. The aim study was develop protocols collection storage “dried blood spot” (DBS) samples, as well validation quantitative determination in whole blood, using this technology subsequent drug monitoring. Materials methods. analyze method detail identify characteristic features taking storing biosamples, analysis scientific literature over past 10 years has been conducted. search materials out from open accessible sources located libraries institutions, electronic databases engines: Elibrary, PubMed, Scopus, Cyberleninka, Medline, ScienceDirect, Web Science, Google Scholar. Primary taking, analyzing samples drop” have prepared. obtain adequate quality developed tested optimized at stages selection storage. By high-performance liquid chromatography mass spectrometric detection (HPLC-MS/MS), sample preparation, ensure that acceptable characteristics were achieved, performed. Results. collection, revealed. Such spot volume effect, hematocrit droplet uniformity, can affect results HPLC-MS/MS analysis, determined. For successful use new methods, appropriate finger adult patients heel newborns, validating these Conclusion. application newly relieves existing constraints conducting TDM, later become promising preclinical clinical studies.